Nicolas L. Bray, Harold Pimentel, Páll Melsted, and Lior Pachter
[Blog note: This paper represents a significant advance in RNA sequencing methodology. The paper is beautifully written and accessible for someone not in the field of bioinformatics. Lior is covering the paper on his blog.]
We present a novel approach to RNA-Seq quantification that is near optimal in speed and accuracy. Software implementing the approach, called kallisto, can be used to analyze 30 million unaligned RNA-Seq reads in less than 5 minutes on a standard laptop computer while providing results as accurate as those of the best existing tools. This removes a major computational bottleneck in RNA-Seq analysis.